CHI3L1 Rabbit Polyclonal Antibody

SKU: orb10365

Featured

Description

Images & Validation

−

Item 1 of 5

Tested Applications	ICC, WB
Dilution range	WB=1:500-2000, ICC/IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human, Mouse

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from rat CHI3L1 (330-381/381aa)
Target	CHI3L1
Molecular Weight	42 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

−

CHI3L1; 39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP39; chitinase 3 like 1(cartilage glycoprotein 39); chitinase 3 like 1; Chitinase 3 like protein 1 precursor; chitinase; GP 39; GP39; HC gp39; HCGP 3P; HCgp39; YKL 40; YKL40; CGP-39; CH3L1_HUMAN; chitinase 3 like 1; Chitinase-3-like protein 1 hCGP-39; YKL-40; YYL 40.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protein: Liver (Rat) lysate at 30 ug, Colon carcinoma (Human) lysates at 30 ug, Primary: Anti-CGP-39/CHI3L1/YKL-40 (orb10365) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, Predicted band size: 42kD, Observed band size: 39kD.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Rat Lung tissue lysates, Primary: Anti-CHI3L1 (orb10365) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 42 kDa.

Tissue/Cell: human endometrium carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated (orb10365) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated (orb10365) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

U87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (orb10365) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

CHI3L1

UniProt

Q9WTV1

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ICC

Immunocytochemistry

View Protocol

Find More Protocols