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Chicken Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit

SKU: orb1496464
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Description

The Chicken Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit is designed to quantify Heat Shock 27kDa Protein 1 (HSPB1) in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 1.57-100 ng/mL and a sensitivity of 0.6 ng/mL.

Research Area

Neuroscience

Images & Validation

Application Notes
standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken HSPB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken HSPB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken HSPB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken HSPB1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityGallus
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range1.57-100 ng/mL
Sensitivity0.6 ng/mL
Concentration100 ng/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
Quality Guarantee

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UniProt Details

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Protocol Information

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48 T
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