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Chicken IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed
SKU: orb346927
Description
Images & Validation
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Item 1 of 2
| Tested Applications | DOT, FC, FLISA, IF, WB |
|---|---|
| Dilution Range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Gallus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Donkey |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Chicken IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Donkey Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Donkey anti-Chicken IgG Antibody Fluorescein Conjugation, Donkey anti-Chicken IgG FITC Conjugated Antibody, Donkey anti-Chicken IgY Antibody Fluorescein Conjugation, Donkey anti-Chicken IgY FITC
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Chicken IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed [orb346889]
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Quality Guarantee
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
DOT
Dot Blot
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