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Chicken IgG (H&L) Secondary Antibody Biotin Conjugated
SKU: orb346919
Description
Images & Validation
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Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000 |
| Reactivity | Gallus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Chicken IgY / IgG whole molecule was produced by repeated immunization with Chicken IgY / IgG whole molecule in rabbit. |
| Purity | Biotin Conjugated Anti-Chicken IgG / IgY secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG / IgY coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Chicken IgG / IgY. Some light chain cross reactivity may be observed against chicken immunoglobulins. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Rabbit anti-Chicken IgG Biotin Conjugated Antibody, Rabbit anti-Chicken IgG Antibody Biotin Conjugation, Chicken Secondary Antibody, Rabbit anti-Chicken IgY Antibody Biotin
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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