Chicken Immunoglobulin of Yolk (IgY) ELISA Kit

SKU: orb866968

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Description

Sandwich Gallus IgY ELISA Kit

Research Area

Hematology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 400 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken IgY protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IgY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IgY in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Gallus
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	6.25-400 µg/mL
Sensitivity	1.81 µg/mL
Concentration	400 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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Immunoglobulin Yolk ELISA Kit

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Chicken Immunoglobulin of Yolk (IgY) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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