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Chloroquine

SKU: orb1298711

Description

Chloroquine

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Pharmacology & Drug Discovery, Protein Biochemistry

Images & Validation

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Chloroquine

Key Properties

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CAS Number54-05-7
MW319.87
Purity99.94% (May vary between batches)
FormulaC18H26ClN3
SMILESCCN(CC)CCCC(C)Nc1ccnc2cc(Cl)ccc12
TargetTLR|||Antibiotic|||HIV Protease|||SARS-CoV|||Parasite|||Autophagy
SolubilityH2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.25 mg/mL (16.41 mM);Ethanol:100 mg/mL (312.63 mM);DMSO:45 mg/mL (140.68 mM)

Bioactivity

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Target IC50
SARS-CoV:8.8 ± 1.2 μM|HIV-1:~6 μg/mL
In Vivo
METHODS: To investigate the effects of Chloroquine on acute liver injury and its potential molecular mechanisms, a single dose of Chloroquine (5-50 mg/kg) was administered intraperitoneally to C57BL/6 mice 2-24 h before CCl4 (10 mL/kg) injection. RESULTS: Chloroquine pretreatment significantly inhibited CCl4-induced acute liver injury, as evidenced by a decrease in serum aminotransferases, aspartate aminotransferase, and a decrease in the histological score of liver injury, and down-regulated CCl4-induced high-mobility histone 1 (HMGB1) expression in liver tissues as well as the levels of serum HMGB1, IL-6, and TNF-α. levels. METHODS: To investigate the relationship between Chloroquine and retinopathy, Chloroquine (50 mg/kg ) was administered intraperitoneally to C57/BL6 mice three times a week for six weeks. RESULTS: Chronic administration of Chloroquine induced retinopathy in mice. mRNAs for IL-1β mRNA, a component of inflammatory vesicles, and caspase1 were increased in the retinas of Chloroquine-treated mice, consistent with the initiation of inflammatory vesicles, and NTPDase1 was increased, suggesting an increase in extracellular ATP in the retina.
In Vitro
METHODS: Human cholangiocarcinoma cells QBC939 were treated with Chloroquine (1.2-200 µM) for 24 h. Cell growth inhibition was detected by MTT. RESULTS: Chloroquine dose-dependently inhibited the cell growth of HRECs with an IC50 of 53.01 µM. METHODS: Human non-small cell lung cancer cells A549 were treated with Chloroquine (10-80 μM) for 24 h. The expression levels of target proteins were detected by Western Blot. RESULTS: Chloroquine induced an increase in LC3-II expression and a decrease in LC3-I expression, resulting in an increase in the LC3-II/LC3-I ratio. The highest LC3-II/LC3-I ratio was observed with 40 μM Chloroquine treatment. METHODS: Human osteosarcoma cells U2OS and human cervical cancer cells HeLa were treated with Chloroquine (100 μM) for 5 h. LAMP1, a marker protein for late endosomal compartment and lysosome, was detected by Immunofluorescence. RESULTS: Chloroquine increased the area of LAMP1 positive structures.

Storage & Handling

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Storage-20°C
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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COVID-19, CQ, Chloroquine, Antibiotic, arthritis, autophagy, Autophagy, Parasite, immune-modulating, inflammatory, infection, Inhibitor, inhibit, Human immunodeficiency virus, malaria, HIV-1, HIV Protease, HIV, HIVProtease, SARS coronavirus, SARS-COV-2, SARSCoV, SARS-CoV, Toll-like Receptor (TLR), rheumatoid, TLR

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Quality Guarantee

Quality Guarantee

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Compound Identifiers

PubChem CID
2719 ↗

Key Properties

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No computed properties available.

Protocol Information

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Available Sizes

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1 ml x 10 mM (in DMSO)
$ 80.00
100 mg
$ 90.00
500 mg
$ 190.00
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