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CHRNA5 Antibody

SKU: orb1539167

Description

CHRNA5 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000), WB (1 - 3 µg/ml)
ReactivityHuman, Monkey
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kD band observed in lysates of cell line KELLY (calculated MW of 53.1kD according to NP_000736.2). Recommended concentration: 1-3 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-HVDRYFTQKEET, from the internal region of the protein sequence according to NP_000736.2.
TargetCHRNA5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CHRNA5, Acra-5, AChR-alpha5, LNCR2, NAChR alpha 5, NACHRA5

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

CHRNA5 Antibody

CHRNA5 antibody (1 ug/ml) staining of KELLY lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CHRNA5 Antibody (orb1539167)

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Available Sizes

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50 μg
¥ 8,710.00
DispatchUsually dispatched within 2-3 weeks
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