Cleaved LC3A Antibody

SKU: orb1933527

Description

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 7

Tested Applications	ICC, IF, IHC-P, WB
Dilution range	WB - 1:1000, IHC-P - 1:100-500, ICC - 1:10-50
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Quality Guarantee

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Western blot analysis of anti-cleaved-LC3 (APG8a) Pab in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified Pab.

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (upper) or Beta-actin (lower).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse mouse embryonic fibroblasts cell line) cells labeling MAP1LC3A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling MAP1LC3A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Immunofluorescence image showing vesicles staining on Hela cell line.The nuclear counter stain is DAPI (blue). The right image is Hela cells treated with Chloroquine 50μM for 16 h.