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CNPase Recombinant Mouse Monoclonal Antibody
SKU: orb1152051
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Description
Research Area
Neurodegenerative Disease, Neuroglia, Neurology, Neuroscience, Oligodendrocyte Marker
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Clone No. | 7A2 |
| Immunogen | KLH conjugated synthetic peptide derived from human CNPase |
| Target | CNP |
| Molecular Weight | 48 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−2', 3'-cyclic-nucleotide 3'-phosphodiesterase; CNPase; Cnp; Cnp-1; Cnp1; 2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 2'3'-cyclic-nucleotide 3'-phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1.
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: 1: HeLa, 2: HEK-293, 3: Rat cerebellum, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 48 kDa, Observed MW: 48 kDa.
Cell line: SH-SY5Y, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152051.
Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-CNPase (orb1152051) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 46 kDa.
SHSY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CNPase) polyclonal Antibody, Unconjugated (orb1152051) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
CNP
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
CNPase Recombinant Mouse Monoclonal Antibody (orb1152051)
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