Complement C3d fragment Rabbit Polyclonal Antibody

SKU: orb156424

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Description

Research Area

Neurology, Synapse Markers

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Complement C3d fragment (1002-1100/1663aa)
Target	C3
Molecular Weight	33/109/181 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Acylation stimulating protein; ASP; C3; C3a anaphylatoxin; Complement C3 alpha chain; Complement C3; Complement C3 precursor; Complement component 3; Complement component C3; Complement factor 3; Plp; Complement C3d fragment; CO3_HUMAN.

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Complement C3d fragment Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Complement C3d fragment) Polyclonal Antibody, Unconjugated (orb156424) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T (Human) cell Lysate at 40 ug, Primary: Anti-Complement C3d fragment (orb156424) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 33'109'181kD, Observed band size: 33 kD.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human U87MG cell lysates, Lane 3: Human A431 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human A549 cell lysates, Primary: Anti-Complement C3d fragment (orb156424) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33/109/187 kDa, Observed band size: 75 kDa.

Quick Database Links

Gene Symbol

UniProt

P01024

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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