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CFB Antibody
SKU: orb1258785
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 80-420 of human Complement Factor B (NP_001701.2). |
| Target | CFB |
| Molecular Weight | Observed: 86kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, Complement factor B Ba fragment, Complement factor B Bb fragment, CFB, BF, BFD
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Western blot analysis of extracts of various cell lines, using Complement Factor B antibody (orb1258785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human mammary cancer using Complement Factor B antibody (orb1258785) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using Complement Factor B antibody (orb1258785) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Complement Factor B antibody (orb1258785) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of Raw264.7 cells using Complement Factor B Polyclonal Antibody (orb1258785) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
CFB
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
CFB Antibody (orb1258785)
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