Cryptotanshinone

SKU: orb1304987

Description

Cryptotanshinone, a bioactive diterpenoid isolated from Salvia miltiorrhiza, acts as a STAT3 inhibitor with an IC50 of 4.6 µM. It exhibits antitumor properties and has been utilized in both in vitro and in vivo cancer research studies to investigate oncogenic signaling pathways.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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CAS Number	35825-57-1
MW	296.36
Purity	99.92% (May vary between batches)
Formula	C₁₉H₂₀O₃
SMILES	C[C@H]1COC2=C1C(=O)C(=O)c1c3CCCC(C)(C)c3ccc21
Target	Autophagy,STAT
Solubility	DMSO:4.17 mg/mL (14.07 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:0.42 mg/mL (1.42 mM)

Bioactivity

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Target IC50	STAT3:4.6 μM
In Vivo	In DU145 cells, Cryptotanshinone treatment (7 μM) for 30 minutes significantly inhibits the phosphorylation of STAT3 at Tyr705 without affecting the phosphorylation at Ser727. After 4 hours, it markedly inhibits the phosphorylation of JAK2 (IC50: 5 μM) but does not affect the phosphorylation of downstream kinases c-Src and EGFR. This suggests that the inhibition of STAT3 phosphorylation at Tyr705 is due to binding to the STAT3 SH2 domain. Cryptotanshinone, a natural product isolated from the root of Salvia miltiorrhiza, significantly inhibits STAT3-dependent luciferase activity, blocks phosphorylation at Tyr705, and dimerization of STAT3. It notably suppresses the proliferation of the DU145 cell line, which possesses constitutively active STAT3 (GI50: 7 μM), by inhibiting STAT3 activity, leading to the downregulation of cyclin D1, Bcl-xL, and anti-apoptotic genes, and subsequently causing cell accumulation in the G0-G1 phase. Cryptotanshinone exhibits mild inhibitory effects on the growth of PC3, LNCaP, and MDA-MB-468 cells. Read more...
In Vitro	In ob/ob mice (C57BL/6J-Lepob), db/db mice (C57BL/KsJ-Leprdb), and ZDF rats, treatment with Cryptotanshinone (600 mg/kg/day) for 3 days significantly reduced blood glucose levels, maintaining this reduction throughout the monitoring period. In ob/ob mice (C57BL/6J-Lepob) and diet-induced obese mice, Cryptotanshinone significantly reduced body weight and food intake in a dose-dependent manner. It also notably decreased fat in adipose tissues and significantly lowered serum cholesterol and triglyceride levels, with AMPK activity in skeletal muscle being 2.5-3 times higher than that in the control mice. Read more...
Cell Research	Cells are exposed to Cryptotanshinone for 24 or 48 hours. For the determination of cell proliferation, the cell proliferation reagent WST-1 is added and WST-1 formazan is quantitatively measured at 450 nm using an ELISA reader.(Only for Reference) Read more...

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cryptotanshinon, Cryptotanshinone, Autophagy, 4733-35-1, Inhibitor, inhibit, Tanshinone c, STAT3, STAT

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Compound Identifiers

PubChem CID

160254 ↗

Key Properties

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No computed properties available.

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