Cyclin B1 Antibody / CCNB1

SKU: orb749862

Description

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Images & Validation

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Tested Applications	IF, IHC-P
Dilution range	Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the SLAMF5 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	CCNB1/1098
Immunogen	Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cyclin B1 antibody (CCNB1/1098).

IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin B1 antibody.

Immunofluorescent staining of human MCF7 cells with Cyclin B1 antibody (clone CCNB1/1098, red), phalloidin (actin, green) and Hoechst (nuclei, blue).

SDS-PAGE analysis of purified, BSA-free Cyclin B1 antibody (clone CCNB1/1098) as confirmation of integrity and purity.

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UniProt

P14635

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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