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Cyclin B1 Mouse Monoclonal Antibody (FITC)
SKU: orb464152
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF |
|---|---|
| Dilution range | IF=1:500-1000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 7D1 |
| Immunogen | KLH conjugated synthetic peptide derived from human Cyclin B1 |
| Target | CCNB1 |
| Purification | Affinity purified by Protein G |
| Conjugation | FITC |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
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100 μl
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Blank control: A431. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Hela. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Jurkat. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: U251. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Tissue/Cell: A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal mouse serum) at 37°C for 20 min, Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (orb464152) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: U251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal mouse serum) at 37°C for 20 min, Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (orb464152) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
CCNB1
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Cyclin B1 Mouse Monoclonal Antibody (FITC) (orb464152)
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