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CCNB1 Antibody
SKU: orb1259192
Featured
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1). |
| Target | CCNB1 |
| Molecular Weight | Observed: 55kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−CCNB1, CCNB
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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (orb1259192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using Cyclin B1 antibody (orb1259192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Cyclin B1 antibody (orb1259192). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (orb1259192) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
CCNB1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
CCNB1 Antibody (orb1259192)
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