Cyclin B1 (phospho Ser126) rabbit pAb

SKU: orb764169

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Description

Rabbit polyclonal antibody to Cyclin B1 (phospho-Ser126)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser126. AA range:91-140
Molecular Weight	60
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-CCNB1 antibody, anti-CCNB antibody, anti-G2/mitotic-specific cyclin-B1 antibody

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Western Blot analysis of various cells using Phospho-Cyclin B1 (S126) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin B1 (Phospho-Ser126) Antibody.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin B1 (Phospho-Ser126) Antibody. The picture on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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