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Cyclin E1 Antibody / CCNE1
SKU: orb1151663
Description
Images & Validation
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| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | Rabbit IgG |
| Clone No. | CCNE1/4935R |
| Immunogen | A portion of amino acids 10-176 was used as the immunogen for the recombinant CCNE1 antibody. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Western blot testing of human HCT-116 cell lysate using recombinant CCNE1 antibody (clone CCNE1/4935R). Predicted molecular weight ~47 kDa.
IHC staining of FFPE human endometrium with recombinant CCNE1 antibody (clone CCNE1/4935R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma tissue with recombinant CCNE1 antibody (clone CCNE1/4935R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CCNE1 antibody (clone CCNE1/4935R) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
Cyclin E1 Antibody / CCNE1 (orb1151663)
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