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Recombinant Keratin 20 Antibody / CK20 / Cytokeratin 20
SKU: orb1151515
Description
Research Area
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | Immunohistochemistry (FFPE): 2-4ug/ml,Western blot: 2-4ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | Rabbit IgG, kappa |
| Clone No. | KRT20/4879R |
| Immunogen | A portion of amino acids 196-323 was used as the immunogen for the recombinant Keratin 20 antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant Keratin 20 Antibody / CK20 / Cytokeratin 20 [orb2636660]
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IHC staining of FFPE human colon tissue with recombinant Keratin 20 antibody (clone KRT20/4879R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma tissue with recombinant Keratin 20 antibody (clone KRT20/4879R) at 2 ug/ml. Inset: PBS instead of primary Ab to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Keratin 20 antibody (clone KRT20/4879R) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
Recombinant Keratin 20 Antibody / CK20 / Cytokeratin 20 (orb1151515)
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