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Description
Research Area
Cell Biology, Stem Cell & Developmental Biology
Images & Validation
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Item 1 of 3
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (2-4ug/ml);,Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | OV-TL12/30 |
| Immunogen | OTN 11, ovarian carcinoma cell line |
| Target | KRT7 |
| Source/Expression System | Bioreactor |
| Molecular Weight | 55kDa |
| Purification | Protein G |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. |
| Concentration | Purified Ab conjugated to Biotin |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-CK-7 antibody, anti-K2C7 antibody, anti-Keratin 55K Type II Cytoskeletal antibody, anti-Keratin Simple Epithelial Type 1 K7 antibody, anti-Keratin Type II Cytoskeletal 7 antibody, anti-Krt2-7 antibody, anti-KRT7 antibody, anti-Sarcolectin antibody, anti-SCL antibody, anti-Type II Mesothelial Keratin K7 antibody, anti-Type-II Keratin Kb7 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
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