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Cytokeratin 8 (phospho Ser432) rabbit pAb Antibody
SKU: orb768905
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser432. AA range:401-450 |
| Molecular Weight | 53 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-KRT8 antibody, anti-CYK8 antibody, anti-Keratin antibody, anti- type II cytoskeletal 8 antibody, anti-Cytokeratin-8 antibody, anti-CK-8 antibody, anti-Keratin-8 antibody, anti-K8 antibody, anti-Type-II keratin Kb8 antibody
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S432) Polyclonal Antibody diluted at 1:1000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Keratin 8 (Phospho-Ser432) Antibody.
Immunofluorescence analysis of HeLa cells, using Keratin 8 (Phospho-Ser432) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Keratin 8 (Phospho-Ser432) Antibody. The picture on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Cytokeratin 8 (phospho Ser432) rabbit pAb Antibody (orb768905)
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