PP65 Antibody / CMV-p65 / Cytomegalovirus

SKU: orb2638051

Description

Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, cytomegalovirus is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of cytomegalovirus disease. An indicator of active CMV infection is needed to facilitate the diagnosis of cytomegalovirus disease in patients with AIDS or HIV infection. Cytomegalovirus p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease.

Images & Validation

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Tested Applications	ELISA, ICC, IF
Dilution range	ELISA (order BSA/sodium azide-free format for coating),Immunofluorescence: 1-3ug/ml
Reactivity	Virus
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PP65 antibody to be titered up or down for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	CMV101
Immunogen	Recombinant Cytomegalovirus p65 protein was used as the immunogen for this PP65 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1X PBS, pH 7.4
Disclaimer	For research use only

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Protocol Information

Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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