Dihydrofluorescein diacetate

SKU: orb1940631

Description

Dihydrofluorescein diacetate is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.(In Vitro):Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence sharply delineates filaments that correspond in all detail to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix. Dihydrofluorescein diacetate may be a useful quantitative method to measure the oxidation potential of nanoparticle-treated cells. Dihydrofluorescein diacetate shows fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium.

Images & Validation

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Key Properties

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CAS Number	35340-49-9
MW	418.401
Purity	>98% (HPLC)
Formula	C₂₄H₁₈O₇
SMILES	CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c1ccccc1C(O)=O
Target	S1P
Solubility	In Vitro: DMSO : ≥ 106.5 mg/mL (254.54 )

Bioactivity

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In Vitro

Dihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizing species. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium. Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix. Dihydrofluorescein diacetate could be an useful and quantitative method for measuring the oxidative potential of nanoparticle-treated cells.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Diacetyldihydrofluorescein

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Gene Symbol

S1P

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