Direct qPCR ProbesMaster

Direct qPCR ProbesMaster is designed for quantitative real-time analysis of target DNA directly from blood, swabs and animal- or plant tissue. The mix allows robust amplification avoiding the requirement of any prior DNA purification procedures.\nThe mix is recommended for use with dual-labeled fluorescent probes, e.g. TaqMan, Molecular Beacon or Scorpion probes. It provides a powerful tool for multiplex-quantification of sample DNA in a broad dynamic range with exceptional sensitivity and precision.\nThe mix contains all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2 x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system.\nThe mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for:\nInterference of remaining components from sample matrix\nDue to the fast and easy but relatively rough sample preparation remaining components from the sample matrix may be co-transferred into the PCR assay. These remains are mostly blocked by a combination of specially optimized additives. If inhibition of the PCR reaction occurs with higher volumes of transferred sample volume, please reduce the sample volumes or use a dilution of the sample in 1x Direct Extraction Buffer or water.\nRemaining components of the sample matrix may also show fluorescence signals specially in the yellow and red spectral range. If using this fluorescence range for multiplex PCR assays or if using ROX, please take special attention that there is no interference between fluorescence from remaining matrix material and the used fluorescence channels for amplicon detection.\nROX Reference Dye\nThe mix can also be used in combination with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.