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Direct Violet 1

SKU: orb1306775

Description

Direct Violet 1 (CCRIS 2413) is a synthetic dye used as a biological stain in research. It is applied in vitro for histological staining and has been utilized in studies investigating dye diffusion and tissue morphology.

Research Area

Infectious Disease & Virology

Images & Validation

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Direct Violet 1

Key Properties

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CAS Number2586-60-9
MW728.66
Purity≥98%
FormulaC32H22N6Na2O8S2
SMILESO=S(C1=CC(O)=C2C(N=NC3=CC=C(C4=CC=C(N=NC5=C(N)C=CC6=CC(S(=O)([O-])=O)=CC(O)=C56)C=C4)C=C3)=C(N)C=CC2=C1)([O-])=O.[Na+].[Na+]
TargetSARS-CoV
SolubilityDMSO:55 mg/mL (75.48 mM)

Bioactivity

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Target IC50
SARS-CoV-2-S-RBD binding to ACE2:1.47 µM (IC50)|SARS-CoV-S1S2 binding to ACE2:2.63 µM (IC50)|TNF-R1-TNFα binding:2.11 µM (IC50)
In Vitro
1. Solution preparation 1. Solution preparation: Dissolve Direct Violet 1 in an appropriate amount of water, usually at a concentration of 0.5-2% (adjust according to the desired depth of dyeing). 2. Working solution preparation: Dissolve Direct Violet 1 in an appropriate amount of solvent (such as DMSO or water) to the desired concentration, usually 1-10 μM. 2. Textile dye application 1. Immerse the fabric in the dye solution and use conventional textile dyeing procedures, usually heating to 60-90°C, and the dyeing time is adjustable (usually 30-60 minutes). 2. During the dyeing process, pH adjustment and temperature control can be used to optimize dye adsorption. 3. Washing and fixing: 1) After dyeing, wash the fabric with water to remove excess dye. 2) A fixing agent can be used as needed to improve the durability and color stability of the dye. III. PPI Inhibition of SARS-CoV-2 Spike Protein and ACE2 Receptor 1. Mix SARS-CoV-2 spike protein and ACE2 receptor in vitro and set different concentrations of Direct Violet 1. 2. Measure the inhibitory effect of Direct Violet 1 on protein-protein interaction using surface plasmon resonance (SPR), fluorescence resonance energy transfer (FRET) or other interaction detection techniques. 3. IC50 determination: By comparing the effects of different concentrations of Direct Violet 1, calculate its IC50 value (1.47-2.63 μM) for inhibiting the interaction between SARS-CoV-2 spike protein and ACE2 receptor. 4. Application: 1) As a tool to study the interaction between SARS-CoV-2 spike protein and ACE2 receptor. 2) As a potential candidate molecule for anti-SARS-CoV-2 drugs.
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Storage & Handling

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Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Inhibitor, inhibit, NSC-75778, NSC75778, NSC 75778, Direct Violet 1, Direct Violet N, Direct Violet1, Direct Violet-1, COVID-19, CCRIS-2413, CCRIS2413, CCRIS 2413, ACE2, Airedale Violet ND, antiviral, SARSCoV, SARS-CoV, SARS coronavirus, TNFα, TNF-R1, pike

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    C32H22N6Na2O8S2

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID
135462983 ↗

Key Properties

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No computed properties available.

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Direct Violet 1 (orb1306775)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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1 g
¥ 910.00
1 ml x 10 mM (in DMSO)
¥ 910.00
DispatchUsually dispatched within 3-5 working days
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