Donkey Goat IgG (heavy and light chains), conjugated with TRITC Antibody

Tetramethylrhodamine (TMR) and its isothiocyanate derivative-conjugated IgG fraction of polyclonal donkey antiserum to goat IgG, heavy and light chains which helps in the identification and measurement of IgG, antigen or antibody at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of goat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fir histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable no-precipitating antibody-binding assays between 1: 1,000 and 1: 10,000.