Doxycycline hyclate

SKU: orb1225539

Description

Doxycycline Hyclate is a synthetic tetracycline derivative with similar antimicrobial activity.(In Vitro):Doxycycline hyclate (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations.Doxycycline hyclate (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells.Doxycycline hyclate (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines.Doxycycline hyclate (0-250 μM, 72 h) inhibits cell viability of breast cancer cells .(In Vivo):Doxycycline hyclate (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner.

Research Area

Pharmacology & Drug Discovery

Images & Validation

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Key Properties

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CAS Number	24390-14-5
MW	512.9
Purity	>98% (HPLC)
Formula	C₂₂H₂₄N₂O₈·HCl·1/2C₂H₆O·1/2H₂O
SMILES	CCO.C[C@@H]1[C@H]2[C@@H]([C@H]3[C@@H](C(=O)C(=C([C@]3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.C[C@@H]1[C@H]2[C@@H]([C@H]3[C@@H](C(=O)C(=C([C@]3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.O.Cl.Cl
Target	ribosome
Solubility	Water: 100 mg/mL (194.95 mM); DMSO: 100 mg/mL (194.95 mM)

Bioactivity

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In Vivo

Doxycycline hyclate (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner. Animal model: 6-month-old female Heterozygous Col3a1-deficient (HT) mice. Dosage: 200 or 800 mg/kg. Administration: Oral gavage; 200 or 800 mg/kg; once daily; 3 months. Result: Reduced MMP-9 activity in a dose-dependent manner.

In Vitro

Doxycycline hyclate (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations. Doxycycline hyclate (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells. Doxycycline hyclate (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines. Doxycycline hyclate (0-250 μM, 72 h) inhibits cell viability of breast cancer cells. Cell Viability Assay Cell line: LNT-229, G55, and U343 glioma cells. Concentration: 0.01, 0.1, 1 or 10 μg/mL Incubation time: 4 days. Result: Affected growth of glioma cells only under high concentrations (10 μg/mL). Cell Viability Assay Cell line: SVG cells. Concentration: 0.01, 0.1, 1 or 10 μg/mL Incubation time: 24 hours. Result: Decreaseed MT-CO1 protein content with concentrations of 1 μg/mL and higher. Cell Proliferation Assay Cell line: MCF 12A, 293T cells. Concentration: 100 ng/mL, 1 μg/mL. Incubation time: 96 hours. Result: Caused reduced proliferation of MCF 12A and 293T cells at 1 μg/mL. Cell Viability Assay Cell line: MCF-7, MDA-MB-468 cells. Concentration: 0-250 μM. Incubation time: 72 hours. Result: Inhibited breast cancer cells in a dose-dependent manner with IC50 values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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WC 2031

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Gene Symbol

ribosome

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