You have no items in your shopping cart.
KO/KD
Validated
Validated
Description
Images & Validation
−
Item 1 of 9
| Tested Applications | ELISA, KO/KD Validated, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000 - 1:40,000, WB: 1:2,000 - 1:10,000 |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG conjugated proteins. |
| Purity | This affinity purified antibody is directed against the FLAG motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG tag antibody detects over-expressed proteins containing the FLAG epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.09 |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit antibody for the detection of FLAG conjugated proteins, rabbit anti DYKDDDDK
Similar Products
−- Item 1 of 11
- Item 1 of 9
DYKDDDDK Tag (FLAG) Antibody [orb345397]
ELISA, KO/KD Validated, WB
Rabbit
Polyclonal
Unconjugated
25 μl - Item 1 of 2
- Item 1 of 2
- Item 1 of 3
![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396135_1.png)
![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396135_2.png)
![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396143_1.png)
![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396143_2.png)
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Smith, Thomas H. et al. Protease-activated receptor-4 and purinergic receptor P2Y12 dimerize, co-internalize, and activate Akt signaling via endosomal recruitment of β-arrestin J Biol Chem, 292, 13867-13878 (2017)
Available Sizes
Select a size below
Free Secondary Antibody (20 ul)0/0
Please add an antibody product to your cart first.
Usually dispatched within 5-10 working days