E3 ELISA Kit

SKU: orb568152

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Description

E3 ELISA Kit

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Key Properties

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Reactivity	All
Sample Types	Serum,Plasma,Cell Culture Supernatant,Cell or tissue lysate,Other liquid samples
Assay Type	Competitive
Assay Time	2 hours
Range	0.781-50ng/ml
Sensitivity	0.469ng/ml

Procedure & Performance

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Assay Principle	This kit is based on a competitive ELISA detection method. The microtiter plate is pre-coated with target antigen. During the assay, the target antigen in the sample or standard competes with the immobilized target for binding sites on the biotinylated detection antibody specific to the target antigen. After incubation, excess conjugate and unbound sample or standard are removed by washing. HRP–streptavidin (SABC) is then added to each well and incubated, followed by the addition of TMB substrate solution. The enzymatic reaction is terminated by adding an acidic stop solution, and the color change is measured at 450 nm using a microplate reader. The concentration of the target antigen in the samples is determined by comparison with the standard curve and is inversely proportional to the OD450 value.
Kit Components	1. ELISA Microplate 2. Standards 3. Detection Antibody 4. HRP-Streptavidin Conjugate 5. TMB Substrate 6. Dilution buffers 7. Stop Solution 8. Wash Buffer 9. Plate Sealers 10. Manual
Reagent Preparation	1. Wash Buffer: Prepare the 1X Wash Buffer using distilled water according to the manual. 2. Standard: Perform gradient dilution according to the instructions in the manual. 3. Other Concentrated Reagents: Dilute the concentrated reagents using the Dilution Buffers provided in the kit to 1 X working solutions as instructed in the manual. Always use a clean pipette tip for each different solution.
Assay Procedure	This procedure is for reference only. 1. After the kit equilibrates to room temperature, add standards or samples to each well and incubate. 2. Add biotinylated antibody working solution to each well and incubate. 3. Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper. 4. Add streptavidin-HRP working solution to each well and incubate._x000b_5. Discard liquid, add wash buffer to each well, wash the plate five times, and blot dry on clean absorbent paper. 6. Add TMB substrate solution to each well and incubate in the dark. 7. Add stop solution to each well, mix thoroughly, and immediately read OD at 450 nm.
Materials Required	1. Microplate readers 2. Centrifuge 3. Incubator 4. Automated plate washer 5. Single-channel or multi-channel high-precision pipettes 6. Disposable pipette tips 7. Sterile tubes 8. Eppendorf tubes 9. Absorbent paper 10. Loading slots
Precision	Intra-assay Precision (Precision within an assay): CV% < 8% Intra-assay precision was evaluated by testing multiple replicates of samples within the same plate. Inter-assay Precision (precision between assays): CV% < 10% Inter-assay precision was evaluated by testing samples across different plates.
Calculation of Results	1. Average the duplicate readings for each Standard, Control, and Sample, and subtract the mean optical density of the zero Standard. 2. Construct a standard curve by plotting the target concentration on the y-axis against absorbance on the x-axis and draw a curve through the data points. 3. Determine the sample concentration by substituting the OD450 value into the standard curve. For diluted samples, multiply the calculated value by the corresponding dilution factor.
Curve Fitting Softwares	1. Curve Expert 2. Thermo SkanIt RE 3. SciDAVis 4. LabPlot 5. ……

Storage & Handling

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Storage	2 - 8 °C for 12 months
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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E3|Estriol

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Protocol Information

Find More Protocols

Reactivity:	Human
Dynamic Range:	0.32-20 ng/mL
Sensitivity:	0.115 ng/mL

Reactivity:	Human
Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.055 ng/mL

Reactivity:	Human
Dynamic Range:	0.32-20 ng/mL
Sensitivity:	0.125 ng/mL

Reactivity:	Human
Dynamic Range:	0.32-20 ng/mL
Sensitivity:	0.123 ng/mL

Reactivity:	Human
Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.061 ng/mL

E3 ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Procedure & Performance

Storage & Handling

Alternative Names

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Protocol Information

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