EB-3D

SKU: orb1299352

Description

EB-3D is a potent and selective choline kinase alpha 1 (ChoKα1) inhibitor with an IC50 of 1 μM. It exhibits anti-cancer activity and is a valuable tool compound for investigating choline metabolism in oncology research, applicable in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience, Signal Transduction

Images & Validation

−

Key Properties

−

CAS Number	1839150-63-8
MW	644.4
Purity	99.48% (May vary between batches)
Formula	C₃₀H₃₆Br₂N₄O₂
SMILES	[Br-].[Br-].CN(C)c1cc[n+](Cc2ccc(OCCOc3ccc(C[n+]4ccc(cc4)N(C)C)cc3)cc2)cc1
Target	AChR,AMPK,Apoptosis
Solubility	DMSO:50 mg/mL (77.59 mM)

Bioactivity

−

Target IC50	ChoKα1:1 μM
In Vitro	EB-3D exhibits a potent antiproliferative activity in a panel of T-leukemia cell lines and primary cultures of pediatric patients. Moreover, the drug strongly induces apoptosis and more importantly it enhanced T-leukemia cell sensitivity to chemotherapeutic agents, such as dexamethasone and l-asparaginase. In addition, the compound induces an early activation of AMPK, the main regulator of cellular energy homeostasis, by its phosphorylation at residue T712 of catalytic subunit α, and thus repressing mTORC1 pathway, as shown by mTOR S2448 dephosphorylation. The inhibition of mTOR in turn affects the activity of several known downstream targets, such as 4E-BP1, p70S6K, S6 Ribosomal Protein and GSK3 that ultimately may lead to a reduction of protein synthesis and cell death. Read more...
Cell Research	Cell Line:JURKAT, CCRF-CEM, HSB-2, MOLT-16, DNA-41, LOUCY, PEER, ALL-SIL cells. Concentration:0.001, 0.01, 0.1, 1, 10, 100 μM.Incubation Time:72 hours

Storage & Handling

−

Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

choline kinase alpha 1 (ChoKα1), EB 3D, EB3D, EB-3D, dephosphorylation, acute, AMP-activated protein kinase, AMPK, Apoptosis, lymphoblastic, inhibit, lipid, Inhibitor, HepG2, homeostasis, leukemia, tumorigenesis

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID

90467110 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols

EB-3D (orb1299352)

Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

No reviews yet

EB-3D

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

Request a Document

Protocol Information

EB-3D (orb1299352)

Step 1: Enter information below

Step 2: Enter the in vivo formulation

Available Sizes

Email

UK Office

US Office

CN Office

Distributors