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Description
Research Area
Cardiovascular Research
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC-Fr, SBR, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | IgG kappa |
| Clone No. | A2 |
| Immunogen | 2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells. |
| Target | FN1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EDB(+) FN, Extra domain-B containing fibronectin
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Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody A2. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of A2 (orb1671928) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing endoplasmic reticular staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671928- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
FN1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
FN1 Antibody (orb1671928)
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