EGFR Antibody (C-term)

SKU: orb1788111

This product can be custom made

Description

Mouse Monoclonal Antibody (Mab)

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P, WB
Dilution Range	WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Immunogen	This EGFR antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1163-1191 amino acids from the C-terminal region of human EGFR. Antigen Region: 1163-1191 aa.
Target	EGFR (HGNC:3236)
Molecular Weight	134277 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. esophagus section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. esophagus section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

All lanes: Anti-EGFR Antibody (C-term) at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

EGFR (HGNC:3236)

UniProt

P00533

RefSeq (Protein):NP_958441.1, NP_958439.1, NP_005219.2, NP_958440.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_958441.1, NP_958439.1, NP_005219.2, NP_958440.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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