ENaC beta Antibody (APC)

SKU: orb383774

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Description

Mouse monoclonal antibody against ENaC beta conjugated to APC.

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1:1000); IHC (1:150)
Reactivity	Mouse
Application Notes	A 1:1000 dilution of SMC-240 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	7B8
Immunogen	Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
Target	ENaC beta
Molecular Weight	87kDa
Purification	Protein G Purified
Conjugation	APC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Amiloride sensitive sodium channel subunit beta, Amiloride-sensitive sodium channel beta-subunit, Beta ENaC, Beta NaCH, ENaC beta, ENaCB, Epithelial Na(+) channel subunit beta, Epithelial Na+ channel beta subunit, Epithelial Na+ channel subunit beta, Epithelial sodium channel beta 2 subunit, Epithelial sodium channel beta 3 subunit, Nonvoltage gated sodium channel 1 beta subunit, Nonvoltage gated sodium channel 1 subunit beta, Nonvoltage-gated sodium channel 1 beta subunit, SCNEB, SCNN 1B, SCNN1B, Sodium channel nonvoltage gated 1 beta, Sodium channel nonvoltage gated 1 beta (Liddle syndrome)

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Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.

Quick Database Links

Gene Symbol

ENaC beta

UniProt

P37090

NCBI Gene ID

24767

RefSeq (Protein):NP_036780

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_036780

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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