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Description
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB. Antigen Region: 237-264 aa. |
| Target | ENO3 |
| Molecular Weight | 46987 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−ENOB Antibody (Center) [orb1931365]
FC, IHC-P, WB
Bovine, Porcine
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl

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ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from RD cell line, mouse skeletal muscle, rat skeletal muscle tissue lysate (from left to right), using ENOB Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001967.3, NP_001180432.1, NP_443739.3 |
|---|
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Protocol Information
ENOB Antibody (Center) (orb1788251)
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