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CCL24 Antibody
SKU: orb1272261
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 1.1_6C7-2C5 |
| Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human Eotaxin-2 as the immunizing antigen. |
| Target | CCL24 |
| Purification | This IgG1K antibody was purified from ascites fluid followed by Protein A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−MPIF2, SCYA24, C-C motif chemokine 24, CK-beta-6, MPIF-2, Ckb-6
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CCL24 Rabbit Polyclonal Antibody [orb13292]
ELISA, IF, IHC-Fr, IHC-P
Human, Mouse, Rat
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl 
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To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with antigen affinity purified anti-human Eotaxin-2 (orb1272559) as the detection antibody at a concentration of approximately 1.0-2.0 ug/ml.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CCL24 Antibody (orb1272261)
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