Recombinant EpCAM Antibody / Extracellular domain

SKU: orb2640564

Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Canine, Feline, Human
Application Notes	Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rEGP40/1372
Immunogen	A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

UniProt

P16422

UniProt Details

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No UniProt data available

Protocol Information

IHC-P

Immunohistochemistry Paraffin

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Find More Protocols

Applications:	IHC-P, WB
Reactivity:	Canine, Feline, Human
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated

Applications:	IHC-P, WB
Reactivity:	Canine, Feline, Human
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated

Applications:	IHC-P, WB
Reactivity:	Canine, Feline, Human
Species/Host:	Mouse
Clonality:	Recombinant
Conjugation:	Unconjugated

Applications:	IHC-P, WB
Reactivity:	Canine, Feline, Human
Species/Host:	Mouse
Clonality:	Recombinant
Conjugation:	Unconjugated

Applications:	IHC-P
Reactivity:	Canine, Feline, Human
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated