EpCAM Recombinant Rabbit Monoclonal Antibody

SKU: orb704350

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Description

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 6

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	6H5
Immunogen	A synthesized peptide derived from human EpCAM (266-314aa)
Target	EPCAM
Molecular Weight	35 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Ber-Ep4; BerEp4; DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; LYNCH8; M4S1; MIC18; MK-1; MOC-31; TACSTD1; TROP1; CD326; EGP; Ep-CAM; EpCAM1; GA733-2; Ly74; Tacsd1; gp40; EPCAM_HUMAN; EPCAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein (EGP); Epithelial glycoprotein 314 (EGP314 | hEGP314); KS 1/4 antigen; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; M1S2; EPCAM_MOUSE; Epithelial glycoprotein 314 (EGP314 | mEGP314); Protein 289A; EPCAM_RAT; Epithelial glycoprotein 314 (EGP314); Protein D5.7A;

Quality Guarantee

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EpCAM Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (EpCAM) Monoclonal Antibody, Unconjugated (orb704350) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (EpCAM) Monoclonal Antibody, Unconjugated (orb704350) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD34) Monoclonal Antibody, Unconjugated (orb704350) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD34) Monoclonal Antibody, Unconjugated (orb704350) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD34) Monoclonal Antibody, Unconjugated (orb704350) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug, Lane 2: Prostate (Mouse) Lysate at 40 ug, Lane 3: Lung (Rat) Lysate at 40 ug, Primary: Anti-EpCAM (orb704350) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD.

Quick Database Links

Gene Symbol

EPCAM

UniProt

P16422

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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