EphA2 Rabbit Polyclonal Antibody

SKU: orb10598

This product can be custom made

Featured

Description

EphA2 Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

−

Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse
Predicted Reactivity	Bovine, Canine, Human, Monkey, Rat

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human EphA2 (901-976/976aa)
Target	EPHA2
Molecular Weight	105 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

ARCC2; CTPA; CTPP1; CTRCT6; ECK; Myk2; Sek-2; Sek2; EPHA2_HUMAN; EPHA2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; 2.7.10.1; EPHA2_MOUSE; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Sample: Human colon carcinoma lysates Lysate at 30 ug, Intestine (Mouse) Lysate at 30 ug, Primary: Anti-EphA2/Eph receptor A2 (orb10598) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 105kD, Observed band size: 105kD, 52/55kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-EphA2 (orb10598) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kD, Observed band size: 115 kD.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-EphA2/Eph receptor A2 Polyclonal Antibody, Unconjugated (orb10598) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

EPHA2

UniProt

P29317

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

Find More Protocols