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EpoR (phospho Tyr368) rabbit pAb
SKU: orb768012
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Epo-R around the phosphorylation site of Tyr368. AA range:341-390 |
| Molecular Weight | 55 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-EPOR antibody, anti-Erythropoietin receptor antibody, anti-EPO-R antibody
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Western Blot analysis of K562 cells using Phospho-EpoR (Y368) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Epo-R (Phospho-Tyr368) Antibody.
Immunofluorescence analysis of HepG2 cells, using Epo-R (Phospho-Tyr368) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells, using Epo-R (Phospho-Tyr368) Antibody. The lane on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
EpoR (phospho Tyr368) rabbit pAb (orb768012)
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