ERα (phospho Tyr537) rabbit pAb Antibody

SKU: orb768047

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Description

Rabbit polyclonal antibody to ER alpha (phospho-Tyr537)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Tyr537. AA range:501-550
Molecular Weight	66
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-ESR1 antibody, anti-ESR antibody, anti-NR3A1 antibody, anti-Estrogen receptor antibody, anti-ER antibody, anti-ER-alpha antibody, anti-Estradiol receptor antibody, anti-Nuclear receptor subfamily 3 group A member 1 antibody

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ERα (phospho Tyr537) rabbit pAb Antibody

Western Blot analysis of 293 cells using Phospho-ERα (Y537) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells, using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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