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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human HER2 (1201-1255/1255aa) |
| Target | ERBB2 |
| Molecular Weight | 185 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD340; HER-2; HER-2/neu; HER2; MLN 19; MLN-19; NEU; NGL; TKR1; VSCN2; c-ERB-2; c-ERB2; p185(erbB2); Erbb-2; c-erbB2; c-neu; l11Jus8; mKIAA3023; HER2/neu; ERBB2_HUMAN; ERBB2; Metastatic lymph node gene 19 protein (MLN 19); Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; 2.7.10.1; MLN19; ERBB2_MOUSE; Kiaa3023; ERBB2_RAT; Epidermal growth factor receptor-related protein; p185neu;
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Tissue/Cell: MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HER2) polyclonal Antibody, Unconjugated (orb10372) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
ERBB2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
ERBB2/HER2 Rabbit Polyclonal Antibody (orb10372)
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