Phospho-HER2 (Tyr877) Rabbit Polyclonal Antibody

SKU: orb6147

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Description

Research Area

Cancer Research, Cell Biology, Epidermal Growth Factor, Receptor Tyrosne Kinases, Tumor Biology

Images & Validation

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Tested Applications	ICC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Reactivity	Human, Rat
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human HER2 around the phosphorylation site of Tyr877 TE(p-Y)HA
Target	ERBB2
Molecular Weight	138 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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ErbB 2(phospho Y877); p-ErbB 2(phospho Y877); HER2(phospho-Tyr877); CerbB2; c erb B2; C erbB 2; C-erbB2; CD340; CD340 antigen; Cerb B2/neu protein; Erb B2; erbb2; HER 2; HER 2/neu; Her2/neu; Herstatin; MLN 19; MLN19; NEU; NEU Proto Oncogene; Neuro Glioblastoma Derived Oncogene Homolog; Neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185 ErbB2; p185erbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Receptor tyrosine protein kinase erbB 2; TKR1.

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Phospho-HER2 (Tyr877) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-HER2 (Tyr877)) Polyclonal Antibody, Unconjugated (orb6147) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Tissue/Cell: A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-HER2 (Tyr877)) polyclonal Antibody, Unconjugated (orb6147) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

ERBB2

UniProt

P04626

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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