Ergosta-4,6,8(14),22-tetraen-3-one

SKU: orb1684622

Description

Research Area

Cell Biology, Protein Biochemistry

Images & Validation

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Key Properties

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CAS Number	19254-69-4
MW	392.62
Formula	C₂₈H₄₀O
SMILES	C[C@@]12C(=C3[C@@]([C@]4(C)C(C=C3)=CC(=O)CC4)(CC1)[H])CC[C@@]2([C@@H](/C=C/[C@@H](C(C)C)C)C)[H]
Target	Others
Solubility	DMSO:16 mg/mL (40.75 mM)

Bioactivity

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In Vivo

Ergosta-4,6,8(14),22-tetraen-3-one (10, 20 mg/kg, gavage, for 8 weeks) exhibited certain preventive effects on aristolochic acid I (AAI)-induced early kidney injury in rats. This compound significantly reduced serum creatinine, blood urea nitrogen, urinary protein, and urinary NAG levels, while also alleviating pathological damage in renal tissues .

In Vitro

Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, 15, and 20 μg/ml, for 6, 12, and 24 hours) exhibited significant cytotoxicity against HepG2 cells, with IC50 values decreasing as treatment duration and concentration increased, reaching 15.6, 11.8, and 10.0 μg/ml, respectively . Treatment with Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, and 20 μg/ml, for 12 hours) induced cell cycle arrest at the G2/M phase in HepG2 cells, with the proportion of G2/M phase cells peaking at 12 hours . Treatment with Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, and 20 μg/ml, for 12 hours) significantly increased apoptosis in HepG2 cells, with the proportions of early and late apoptotic cells rising in correlation with the concentration of Ergosta-4,6,8(14),22-tetraen-3-one . Treatment with Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, and 20 μg/ml, for 12 hours) led to the activation of caspase-3, -8, and -9, increased cleavage of PARP, upregulation of Bax protein expression, downregulation of Bcl-2 protein expression, and an elevated Bax/Bcl-2 ratio, indicating that ergone induces apoptosis in HepG2 cells through both intrinsic and extrinsic apoptotic pathways .

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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p53

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Compound Identifiers

PubChem CID

6441416 ↗

Key Properties

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No computed properties available.

Protocol Information

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Purity:	>98% (HPLC)
CAS Number:	19254-69-4
MW:	392.62
Formula:	C₂₈H₄₀O

Ergosta-4,6,8(14),22-tetraen-3-one

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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(22E)-Ergosta-4,6,8(14),22-tetraen-3-one [orb3054205]

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