ERK1 + ERK2 Rabbit Polyclonal Antibody (PE)

SKU: orb499412

Description

Images & Validation

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Item 1 of 1

Tested Applications	FC, IF
Dilution range	Flow-Cyt=1μg/Test, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Goat, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human ERK2 (301-358/358aa)
Target	MAPK3
Purification	Affinity purified by Protein A
Conjugation	PE

Storage & Handling

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Storage	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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ERK 1/2; ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen activated protein kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_HUMAN; MK03_HUMAN; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44MAPK; PRKM 1; PRKM 2; PRKM 3; PRKM1; PRKM2; PRKM3; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 3; Protein tyrosine kinase ERK 2.

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ERK1 + ERK2 Rabbit Polyclonal Antibody (PE)

Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-ERK1 + ERK2 antibody (orb499412), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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Gene Symbol

MAPK3

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Protocol Information

Flow Cytometry

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Immunofluorescence

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