Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human ERN2 (465-550/926aa)
Target	ERN2
Molecular Weight	98 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ER to nucleus signalling 2; ERN 2; hIRE2p; Inositol requiring 1 beta; Ire1 beta; IRE1 S. cerevisiae homolog of; IRE2; Serine/threonine protein kinase/endoribonuclease IRE2; ERN2_HUMAN; Endoplasmic reticulum-to-nucleus signaling 2; Inositol-requiring protein 2 (hIRE2p); Ire1-beta (IRE1b).

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Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-ERN2 antibody (orb100216), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/test. Protocol, The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

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Gene Symbol

ERN2

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Protocol Information

Flow Cytometry

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