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Estrogen Receptor alpha Rabbit Polyclonal Antibody
SKU: orb10612
Featured
Description
Research Area
Epigenetics and Nuclear Signaling
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:50-200 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Mouse, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from the middle of human Estrogen Receptor alpha (331-360/595aa) |
| Target | ESR1 |
| Molecular Weight | 67 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1(alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.
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Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: K562 (Human) Cell Lysate at 30 ug, Lane 3: Liver (Rat) Lysate at 40 ug, Lane 4: Molt-4 (Human) Cell Lysate at 30 ug, Lane 5: Raji (Human) Cell Lysate at 30 ug, Lane 6: Hela (Human) Cell Lysate at 30 ug, Lane 7: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-Estrogen Receptor alpha (orb10612) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 67 kD, Observed band size: 62/50 kD.
Tissue/Cell: MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (orb10612) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (orb10612) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
ESR1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
Estrogen Receptor alpha Rabbit Polyclonal Antibody (orb10612)
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