Fab Goat IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb348429

Description

Fab Goat IgG (H&L) antibody (FITC)

Images & Validation

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Item 1 of 1

Tested Applications	FC, FLISA, IF, WB
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Goat
Application Notes	Fab Anti-Goat IgG Fluorescein Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG Fab
Immunogen	Goat IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum. No reaction was observed against anti-papain or anti-Donkey IgG F(c).
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Donkey Fab Anti-Goat Antibody Fluorescein Conjugation, Donkey Fab Anti-Goat FITC Conjugated Antibody

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Fab Goat IgG (H&L) Antibody Fluorescein Conjugated

Western blot of Fluorescein conjugated Donkey Fab Anti-Goat IgG secondary antibody. Lane 1: Molecular Weight Marker. Lane 2: Goat IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None.

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence