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Fab Human IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb348466

Description

Fab Human IgG (H&L) antibody (FITC)

Images & Validation

Tested ApplicationsFC, FLISA, IF
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
ReactivityHuman
Application Notes
Fab Anti-Human IgG (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

Antibody TypeSecondary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG Fab
ImmunogenHuman IgG whole molecule
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
ConjugationFITC

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration0.5 mg/mL
DisclaimerFor research use only

Alternative Names

Rabbit Fab Anti-Human IgG Antibody Fluorescein Conjugation, Rabbit Fab Anti-Human IgG FITC Conjugated Antibody

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

Fab Human IgG (H&L) Antibody Fluorescein Conjugated (orb348466)

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Available Sizes

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500 μl
¥ 5,720.00
DispatchUsually dispatched within 5-10 working days
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