Fab Mouse IgG (H&L) Antibody

SKU: orb348471

Description

Fab Mouse IgG (H&L) antibody

Images & Validation

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Item 1 of 2

Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:15,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Mouse
Application Notes	Fab Anti-Mouse IgG has been tested by ELISA, dot blot, and SDS-PAGE and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG Fab
Immunogen	Anti-Mouse IgG (H&L) antibody was produced by repeated immunization with mouse IgG whole molecule in goat.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/ml
Disclaimer	For research use only

Alternative Names

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Goat Fab Anti-Mouse IgG Antibody

Quality Guarantee

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Dot Blot results of Goat Fab Anti-Mouse IgG Antibody. Dots are Mouse IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Goat Fab Anti-Mouse IgG at 1 µg/ml for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40000 for 30 min at RT.

SDS-PAGE results of Goat Fab Anti-Mouse IgG (H&L) Antibody. Lane 1: Goat Fab Anti-Mouse - Non-reduced. Lane 2: Opal Prestained Ladder -. Lane 3: Goat Fab Anti-Mouse - Reduced. Load: 1.0ug. Coomassie stained and imaged.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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