Fab Rat IgG (H&L) Antibody Rhodamine Conjugated

SKU: orb348494

Description

Fab Rat IgG (H&L) antibody (TRITC)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, IF
Dilution range	IF: 1:500-1:2,500
Reactivity	Rat
Application Notes	Fab Anti-Rat IgG Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG Fab
Immunogen	Rat IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat Fab Anti-Rat IgG Antibody Rhodamine Conjugation, Goat Fab Anti-Rat IgG TRITC Conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Fab Rat IgG (H&L) Antibody Rhodamine Conjugated

Dot Blot results of Goat Fab Anti-Rat IgG Antibody Rhodamine Conjugate. Dots are Rat IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Goat Fab Anti-Rat IgG Antibody TRITC at 1 ug/ml in orb348637 1hr RT.

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Immunofluorescence

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DOT

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