F(ab')2 Cat IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb348086

Description

F(ab')2 Cat IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:2,000 - 1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
Reactivity	Feline
Application Notes	F(ab')2 Anti-Cat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Cat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Cat IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Cat IgG and Cat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit F(ab')2 Anti-Cat alkaline phosphatase Conjugated Antibody, Rabbit Fab2 Anti-Cat Antibody alkaline phosphatase Conjugation, Rabbit Fab'2 Anti-Cat Antibody alk phos Conjugation

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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